NCIt definition : A preparation of autologous T-lymphocytes (ATL) that have been transduced with the
retroviral vector SFG, a Moloney murine leukemia (Mo-MuLV) virus-based vector, encoding
a chimeric antigen receptor (CAR) composed of a single chain single-chain variable
fragment (scFv) directed against the CD30 antigen (CAR.CD30) and linked, via the spacer
human IgG1 immunoglobulin heavy constant region (hinge-CH2CH3 region), to the co-stimulatory
domains of CD28 and the zeta chain of the TCR/CD3 complex (CD3-zeta) (CD28zeta), with
potential immunostimulating and antineoplastic activities. Upon administration, the
autologous CD30CAR-CD28-CD3zeta-expressing T-lymphocytes specifically recognize and
bind to CD30-expressing tumor cells, resulting in specific T-cell-mediated tumor cell
lysis. CD30, a cell surface receptor and a member of the tumor necrosis factor (TNF)
receptor superfamily, is transiently expressed on activated lymphocytes and is constitutively
expressed in hematologic malignancies.;