NCIt definition : The sulfate salt form of netilimicin, a semisynthetic, water soluble aminoglycoside
antibiotic. Netilmicin is derived from sisomicin, a naturally occurring aminoglycoside
antibiotic produced by the fermentation of Micromonospora inyoensis. Netilmicin irreversibly
binds to the 16S rRNA and S12 protein of the bacterial 30S ribosomal subunit. As a
result, this agent interferes with the assembly of initiation complex between mRNA
and the bacterial ribosome, thereby inhibiting the initiation of protein synthesis.
In addition, netilmicin induces misreading of the mRNA template and causes translational
frameshift, thereby results in premature termination. This eventually leads to bacterial
cell death.;