Preferred Label : Anti-FL(FITC-E2) CAR T Cells;
NCIt synonyms : Fluorescein-specific (FITC-E2)-CAR T Cells; Anti-FL(FITCE2) CAR Expressing T Cells; AntiFL(FITCE2) CAR Expressing T Cells; Anti-FL(FITC-E2) CAR T-lymphocytes;
NCIt definition : A preparation of genetically modified T-cells transduced with a replication incompetent,
self-inactivating (SIN) lentiviral vector expressing a second generation chimeric
antigen receptor (CAR) consisting of an anti-fluorescein (anti-FL) fluorescein isothiocyanate
(FITC)-E2 single chain variable fragment (scFv), that is coupled, via an immunoglobulin
G4 (IgG4) hinge-CH2(L295D)-CH3 spacer, to the costimulatory signaling molecules CD28,
CD137 (4-1BB), and CD3 zeta, and linked to a truncated form of the human epidermal
growth factor receptor (EGFRt), with potential immunostimulating and antineoplastic
activities. Prior to the administration of anti-FL(FITC-E2) CAR T-cells, the CAR-T
adaptor molecule (CAM) EC17 is administered. EC17 is a bispecific molecule that is
composed of folic acid conjugated to FITC (folate-FITC). EC17 targets and binds with
its folate moiety with high affinity to folate receptor (FR)-expressing tumor cells.
Upon administration of the anti-FL(FITC-E2) CAR T-cells, these cells are attracted
by and bind to the FITC antigen moiety of EC17. Upon binding to EC17, the T-cells
induce specific tumor cell lysis, cytokine secretion, and proliferation, and activate
a robust immune response against the EC17-bound, FR-expressing tumor cells. FR is
overexpressed in various tumor cell types and is associated with increased leukemic
cell proliferation and aggressiveness. The co-stimulatory molecules are required for
full T-cell activation and enhance both proliferation of T-cells and antitumor activity.
EGFRt both facilitates detection of the administered T-cells in vivo and can promote
elimination of those cells following a cetuximab-induced antibody-dependent cellular
cytotoxicity (ADCC) response. The reactivity of the anti-FL(FITC-E2) CAR T-cells is
dependent on dosing of EC17, and therefore allows CAR T-cell activity to be controlled
by dosing of EC17.;
NCI Metathesaurus CUI : CL937741;
Origin ID : C158099;
UMLS CUI : C4764048;
Semantic type(s)
- Cell [UMLS semantic type]
- chemical_or_drug_affects_cell_type_or_tissue
- chemical_or_drug_has_mechanism_of_action
- chemical_or_drug_has_physiologic_effect
- concept_is_in_subset
