NCIt definition : A preparation of cells, which consists of autologous marrow infiltrating lymphocytes
(MILs), that are manipulated in vitro, with potential antitumor and immune stimulating
activities. MILs are harvested from autologous bone marrow from multiple myeloma patients
and, in vitro, are exposed to and activated by anti-CD3/anti-CD28 monoclonal antibodies
covalently attached to super-paramagnetic microbeads. After removal of the beads and
expansion of the cells in culture, the activated MILs (aMILs) are re-introduced into
the patient. The aMILs possess enhanced myeloma specificity, and are able to infiltrate
the tumor microenvironment and initiate tumor cell lysis. CD3 and CD28, co-stimulatory
molecules expressed on the surface of T-lymphocytes, play a key role in the activation
of T-cells.;