MeSH definition : A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The
probes are designed to exactly match two adjacent sequences of a specific target DNA.
The chain reaction is repeated in three steps in the presence of excess probe: (1)
heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and
(3) joining of the probes by thermostable DNA ligase. After the reaction is repeated
for 20-30 cycles the production of ligated probe is measured.;
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The
probes are designed to exactly match two adjacent sequences of a specific target DNA.
The chain reaction is repeated in three steps in the presence of excess probe: (1)
heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and
(3) joining of the probes by thermostable DNA ligase. After the reaction is repeated
for 20-30 cycles the production of ligated probe is measured.
Allowable qualifiers