MeSH definition : A technique with which an unknown region of a chromosome can be explored. It is generally
used to isolate a locus of interest for which no probe is available but that is known
to be linked to a gene which has been identified and cloned. A fragment containing
a known gene is selected and used as a probe to identify other overlapping fragments
which contain the same gene. The nucleotide sequences of these fragments can then
be characterized. This process continues for the length of the chromosome.;
A technique with which an unknown region of a chromosome can be explored. It is generally
used to isolate a locus of interest for which no probe is available but that is known
to be linked to a gene which has been identified and cloned. A fragment containing
a known gene is selected and used as a probe to identify other overlapping fragments
which contain the same gene. The nucleotide sequences of these fragments can then
be characterized. This process continues for the length of the chromosome.
Allowable qualifiers