Preferred Label : Michaelis–Menten kinetics;
IUPAC definition : The dependence of an initial rate of reaction upon the concentration of a substrate
S that is present in large excess over the concentration of an enzyme or other catalyst
(or reagent) E with the appearance of saturation behaviour following the Michaelis-Menten
equation: \[\nu \frac{V\ \left[{S}\right]}{K_{{m}} \left[{S}\right]}\] where v is
the observed initial rate, V is its limiting value at substrate saturation (i.e. S
K m), and Km the substrate concentration when v V 2. The definition is experimental,
i.e. it applies to any reaction that follows an equation of this general form. The
symbols V max or v max are sometimes used for V. The parameters V and Km (the 'Michaelis
constant') of the equation can be evaluated from the slope and intercept of a linear
plot of v-1 i vs /i . [S]-1 (a 'Lineweaver–Burk plot') or from slope and intercept
of a linear plot of v i vs /i . v/[S] ('Eadie–Hofstee plot'). A Michaelis–Menten
equation is also applicable to the condition where E is present in large excess, in
which case the concentration E appears in the equation instead of S. The term has
sometimes been used to describe reactions that proceed according to the scheme: \[{E}
{S}\overset{k_{1}}{\underset{k_{-1}}\rightleftarrows }{ES}\overset{k_{{cat}}}{\rightarrow
}{Products}\] in which case Km (k-1 kcat)/k1 (Briggs–Haldane conditions). It has
more usually been applied only to the special case in which k-1 k cat and Km k-1/k1
Ks; in this case Km is a true dissociation constant (Michaelis–Menten conditions).;
Origin ID : M03892;
See also
The dependence of an initial rate of reaction upon the concentration of a substrate
S that is present in large excess over the concentration of an enzyme or other catalyst
(or reagent) E with the appearance of saturation behaviour following the Michaelis-Menten
equation: \[\nu \frac{V\ \left[{S}\right]}{K_{{m}} \left[{S}\right]}\] where v is
the observed initial rate, V is its limiting value at substrate saturation (i.e. S
K m), and Km the substrate concentration when v V 2. The definition is experimental,
i.e. it applies to any reaction that follows an equation of this general form. The
symbols V max or v max are sometimes used for V. The parameters V and Km (the 'Michaelis
constant') of the equation can be evaluated from the slope and intercept of a linear
plot of v-1 i vs /i . [S]-1 (a 'Lineweaver–Burk plot') or from slope and intercept
of a linear plot of v i vs /i . v/[S] ('Eadie–Hofstee plot'). A Michaelis–Menten
equation is also applicable to the condition where E is present in large excess, in
which case the concentration E appears in the equation instead of S. The term has
sometimes been used to describe reactions that proceed according to the scheme: \[{E}
{S}\overset{k_{1}}{\underset{k_{-1}}\rightleftarrows }{ES}\overset{k_{{cat}}}{\rightarrow
}{Products}\] in which case Km (k-1 kcat)/k1 (Briggs–Haldane conditions). It has
more usually been applied only to the special case in which k-1 k cat and Km k-1/k1
Ks; in this case Km is a true dissociation constant (Michaelis–Menten conditions).
