Preferred Label : frequency-domain fluorometry;
IUPAC acronym : DC; UV; AC;
IUPAC definition : Technique that permits recovery of the parameters characterizing a fluorescence decay.
Instead of using an exciting visible, UV, or infrared radiation pulse (see single-photon
timing), the sample is excited by sinusoidally modulated radiation at high frequency.
The fluoresence response is sinusoidally modulated at the same frequency as, but delayed
in phase and partially demodulated with respect to the excitation.;
Scope note : phase and modulation measurements can be done by using either a cw laser (or a xenon
lamp) and an optical modulator (in general a pockel cell) or the harmonic content
of a pulsed laser.; the modulation ratio is defined as the ratio is defined as the ratio of the modulation
depth (ac/dc ratio) of the fluorescence and the modulation depth of the excitation.
the phase shift and the modulation ratio characterize the harmonic response of the
system. these parameters are measured as a function of the modulation frequency. no
deconvolution is necessary because the data are directly analysed in the frequency
domain.;
Origin ID : FT07384;
Automatic exact mappings (from CISMeF team)
- False automatic mappings
- See also
Technique that permits recovery of the parameters characterizing a fluorescence decay.
Instead of using an exciting visible, UV, or infrared radiation pulse (see single-photon
timing), the sample is excited by sinusoidally modulated radiation at high frequency.
The fluoresence response is sinusoidally modulated at the same frequency as, but delayed
in phase and partially demodulated with respect to the excitation.
