Genetic polymorphisms of CXCR5 and CXCL13 are associated with non-responsiveness to the hepatitis B vaccine
Auteur Zhaojun Duan
Auteur Xiangmei Chen
Auteur Zhenglun Liang
Auteur Ying Zeng
Auteur Fengcai Zhu
Auteur Lu Long
Auteur Malcolm A. McCrae
Auteur Hui Zhuang
Auteur Tao Shen
Auteur Fengmin Lu
Volume 32
Numéro 41
Pages 5316-5322
Publication Vaccine
ISSN 1873-2518
Date Sep 15, 2014
Résumé A cohort based study has been undertaken to investigate the possible association of genetic polymorphisms in genes functionally related to follicular T helper (TfH) cells with non-responsiveness to hepatitis B virus (HBV) vaccination. A total of 24 single nucleotide polymorphisms (SNPs) in 6 TfH related genes (CXCR5, ICOS, CXCL13, IL-21, BCL6 and CD40L) were investigated in 20 non-responders and 45 responders to HBV vaccination. Genetic association analysis revealed that three SNPs (rs497916, rs3922, rs676925) in CXCR5 and one SNP (rs355687) in CXCL13 were associated with hepatitis B vaccine efficacy. In addition, significantly unbalanced distributions of two haplotypes, defined by three SNPs (rs497916, rs3922, rs676925) within CXCR5, were also seen between non-responders and responders. Furthermore, we demonstrated that the rs3922 « GG » genotype was associated with higher levels of CXCR5 than the « AG » and « AA » genotype in a group of healthy volunteers. A dual luciferase report assay was used to confirm that the « G » allele in rs3922 may lead to higher gene expression than the « A » allele, implicating that rs3922 might be a functional SNP affecting CXCR5 expression. These results indicated that polymorphism associated changes in CXCR5 expression in TfH cells may be associated with non-responsiveness to hepatitis B vaccination.
Chercher cette référence sur : Google Scholar, Worldcat
doi:10.1016/j.vaccine.2014.07.064
Laisser une réponse
Vous devez etre connectez Pour poster un commentaire