Preferred Label : Tandem Affinity Purification;
MeSH definition : A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES
that have been assembled with a tagged component (bait) that interacts with other
partners in the complex. The interacting bait component is labeled with a dual-affinity
tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification
of only the partners of the complex interacting with the bait by affinity chromatography.;
MeSH synonym : Affinity Purification, Tandem;
Related MeSH term : Affinity Chromatography, Tandem; Chromatography, Tandem Affinity; Tandem Affinity Chromatographies;
Origin ID : D000078791;
UMLS CUI : C4704846;
Allowable qualifiers
- Record concept(s)
- Semantic type(s)
A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES
that have been assembled with a tagged component (bait) that interacts with other
partners in the complex. The interacting bait component is labeled with a dual-affinity
tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification
of only the partners of the complex interacting with the bait by affinity chromatography.
