Endoplasmic Reticulum Degradation Pathway

Preferred Label : Endoplasmic Reticulum Degradation Pathway;

NCIt synonyms : ER Degradation Pathway;

NCIt related terms : ER-associated degradation (ERAD) Pathway;

Alternative definition : BIOCARTA: The endoplasmic reticulum (ER) of the cell operates a quality control system that identifies misfolded proteins, transports them into the cytoplasm and then targets them for degradation by the proteasome. Aberrant protein degradation is the mechanism underlying many diseases, including cystic fibrosis and heritable forms of lung and liver disease. The pathways that orchestrate the destruction of aberrant proteins are collectively termed ER-associated degradation (ERAD). Most of the proteins synthesized in the ER are cotranslationally modified with N (asparagine)-linked glycans by addition of triglycosylated, branched oligosaccharides at asparagines residues. Rapid glucose trimming by ER glucosidases generates monoglucosylated N-glycans (Glc0-3Man9GlcNAc2). Productive folding of the polypeptides to which the glycans are attached requires the services of an ER lectin chaperone called calnexin (CNX) that recognize the Glc1Man9GlcNAc2 oligosaccharide and assists the folding of newly synthesized glycoproteins. Because the half-time of monoglucose in the nascent glycoproteins is about 2 min, the protein should be repeatedly reglycosylated by UDP-glucose: glycoprotein glucosyltransferase (UGGT) resulting in association with CNX. Next, the mannose residue from the middle branch of the Man9GlcNAc2 oligosaccharide is removed by ER -mannosidase I, then correctly folded glycoproteins bearing Man8GlcNAc2 isomer B (Man8B form) are transported out of the ER to the Golgi apparatus. If the native structure of the glycoprotein cannot be achieved it is retained in the ER. When substrates are released from CNX by glucosidase II, they may be transferred to one of 2 pathways: EDEM for degradation or UUGT for reentry into CNX cycle. A glycoprotein with permanent structural defects is modified by the slow-acting enzyme ER mannosidase I. This enzyme attaches Man8 glycans to misfolded glycoproteins and in so doing generates a signal that results in ER-associated glycoprotein degradation (GERAD). A glycoprotein with a glycan in the form Glc0-3Man8GlcNAc2 is released from the calnexin cycle, recognised by a mannosidase- like protein EDEM and dislocated from the ER to the cytosol. Here the protein binds to the glycan-specific F box protein Fbx2 and is targeted to the SCF-Fbx2-type E3 ubiquitin ligase complex. This results in the protein being polyubiquitinated. The protein is subsequently deglycosylated by a cytosolic peptide N-glycanase (PNGase) that is closely associated with the ubiquitin ligase complex and/or the 19S lid of the proteasome. Finally, the ubiquitinated, deglycosylated protein is degraded by the proteasome. (This definition may be outdated - see the DesignNote.);

NCIt note : The BIOCARTA Definition (ALT_DEFINITION) for this pathway concept was provided by BioCarta. This property was not created by, nor is it maintained by the NCI Thesaurus staff. Additionally, BioCarta is no longer updating its pathway data; thus, the BIOCARTA Definition might be outdated or inaccurate. Please see the Terms and Conditions for Use at http://www.biocarta.com/.;

Biocarta ID : h_eradPathway;

Details

Origin ID

C39288;

UMLS CUI

C1512129;

Semantic type(s)
- Molecular Function [UMLS semantic type]
has_gene_product_element
- Calnexin [NCIt concept]
- DNA Damage-Inducible Transcript 3 Protein [NCIt concept]
- E3 Ubiquitin-Protein Ligase RBX1 [NCIt concept]
- SUMO-Activating Enzyme Subunit 1 [NCIt concept]
pathway_has_gene_element
- CANX wt Allele [NCIt concept]
- CUL1 wt Allele [NCIt concept]
- DDIT3 wt Allele [NCIt concept]
- RBX1 wt Allele [NCIt concept]
- SAE1 wt Allele [NCIt concept]

Keyword's position in hierarchy(ies) :

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