Dideoxy Chain Termination DNA Sequencing

Preferred Label : Dideoxy Chain Termination DNA Sequencing;

NCIt synonyms : Sanger Sequencing;

NCIt definition : A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.;

Alternative definition : CDISC: A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs (which are radioactively or fluorescently labeled) are combined within the reaction mixture. Once the polymerase chain reaction is complete the DNA strands are separated by size and the labeled chain terminating dNTPs can be read in sequence by the investigator or a machine.;

Details

Origin ID

C19641;

UMLS CUI

C1511897;

Automatic exact mappings (from CISMeF team)
- Sanger sequencing [OMIConto structure]
- chain termination sequencing [OBI concept]
CISMeF manual mappings
- First generation sequencing analysis [OMIConto structure]
Semantic type(s)
- Molecular Biology Research Technique [UMLS semantic type]
UMLS correspondences (same concept)
- First generation sequencing analysis [OMIConto structure]
concept_is_in_subset
- CDISC SDTM Method Terminology [NCIt concept]
- CDISC SDTM Terminology [NCIt concept]
- Clinical Data Interchange Standards Consortium Terminology [NCIt concept]

Keyword's position in hierarchy(ies) :

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