Preferred Label : Yeast One/Two-Hybrid System;
NCIt synonyms : Yeast Two Hybrid System; Yeast One Hybrid System; Two Hybrid; Yeast One Two-Hybrid System;
NCIt definition : Molecular biology technique used to identify interacting proteins in a yeast model.
A protein of interest (the bait) is fused to the DNA-binding domain of a yeast transcription
factor (usually GAL4). (Usually) a cDNA library is fused to the GAL4 activation domain.
The library is then introduced into yeast cells carrying the bait construct. Functional
interaction of the bait with fusion proteins from the library bring together the binding
and activation domains of the transcription factor, allowing activation of a reporter
gene. (syn: yeast two-hybrid system) (NCI/OSP);
Origin ID : C18140;
UMLS CUI : C0599647;
Automatic exact mappings (from CISMeF team)
- Semantic type(s)
