Preferred Label : Intracellular Cytokine Stain Flow Cytometric Assay;
NCIt synonyms : ICS;
NCIt related terms : Intracellular Cytokine Staining;
NCIt definition : A technique that is used with flow cytometry to measure cytokine production in isolated
cells. Cells are stimulated with an antigen or mitogen, and cytokine secretion is
blocked using protein transport inhibitors. The cells are then stained with fluorochrome
labeled monoclonal antibodies that target surface markers, fixed and permeabilized,
and stained with fluorochrome labeled anti-cytokine monoclonal antibodies prior to
analysis by flow cytometry.;
Alternative definition : CDISC: A technique that is used with flow cytometry to measure cytokine production
in isolated cells. Cells may be stimulated with an antigen or mitogen, and cytokine
secretion may be blocked using protein transport inhibitors. The cells are then stained
with fluorochrome labeled monoclonal antibodies that target surface markers, fixed
and permeabilized, and stained with fluorochrome labeled anti-cytokine monoclonal
antibodies prior to analysis by flow cytometry.;
NCI Metathesaurus CUI : CL1647977;
Origin ID : C178026;
UMLS CUI : C5553936;
Automatic exact mappings (from CISMeF team)
Semantic type(s)
concept_is_in_subset