Preferred Label : Stable Isotope Labeling with Amino Acids in Cell Culture;
NCIt synonyms : Amino Acid-Coded Mass Tags; AACT; Stable Isotope Labeling by Amino Acids in Cell Culture; SILAC; Stable Isotope Labeling by/with Amino Acids in Cell Culture;
NCIt definition : A method for generating labeled proteins or peptides for mass spectrometry analysis
that leverages the cellular protein translation machinery. Briefly, cells are grown
through several rounds of cell division in a culture medium that lacks a standard
essential amino acid and insteady contains a non-radioactive isotope-labeled version
of that amino acid. After a set number of cell doublings, each occurrence of the essential
amino acid will be replaced with the labeled analog.;
NCI Metathesaurus CUI : CL970427;
Origin ID : C161804;
UMLS CUI : C5202706;
Automatic exact mappings (from CISMeF team)
- Semantic type(s)
