Preferred Label : Proximity Ligation Assay;
NCIt synonyms : PLA;
NCIt definition : A technique that leverages immunoassay and DNA amplification technology to detect
protein-protein interactions with high specificity and sensitivity. A sample is prepared
and then exposed to primary antibodies raised in different species recognizing the
target epitopes on the proteins of interest. Then, secondary antibodies that are tagged
with short oligonucleotides and that target each primary antibody are added. Next,
a mixture comprised of a ligase, PCR components and a connector oligonucleotide, which
hybridizes to both oligonucleotide tags and can prime rolling DNA circle synthesis,
are added and the sample is subjected to real-time PCR to amplify the DNA circles.
Rolling circle synthesis and amplification is only possible if the protein epitopes
are in close proximity (i.e. in a protein complex). Finally, a labeled complementary
nucleotide probe is added to detect and/or visualize the amplified DNA.;
NCI Metathesaurus CUI : CL935889;
Origin ID : C156754;
UMLS CUI : C4722316;
Automatic exact mappings (from CISMeF team)
- Semantic type(s)
