NCIt synonyms : Assay for Transposase-Accessible Chromatin Using Sequencing; Assay for Transposase-Accessible Chromatin Sequencing; Assay for Transposase Accessible Chromatin with High-Throughput Sequencing;
NCIt related terms : ATAC-seq (assay for Transposase-Accessible Chromatin (ATAC) strategy is used to study
genome-wide chromatin accessibility);
NCIt definition : A molecular genetic technique that isolates and sequences chromosomal regions that
are rich in open chromatin. First, nuclei are harvested from a cellular sample. Then
a hyperactive Tn5 transposase is added to the nuclei where it excises non-nucleosomal
DNA strands and ligates co-administered high-throughput sequencing adapters (tagmentation).
The tagged DNA fragments are isolated, amplified by PCR and sequenced. The number
of reads for specific region of DNA correlate with increased chromatin accessibility
and this method can identify regions of transcription factor and nucleosome binding.;
Automatic exact mappings (from CISMeF team)