Preferred Label : NOXA BH3 Priming;
NCIt synonyms : PMAIP1 BH3 Priming;
NCIt definition : An assay that indirectly quantitates the expression of the antiapoptotic protein,
induced myeloid leukemia cell differentiation protein Mcl-1 (MCL1; EAT), in a biological
sample. Mitochondria or whole cells are isolated from a patient sample and stained
with JC-1. In healthy cells with high mitochondrial membrane potential, JC-1 forms
aggregates that emit red fluorescence, while in apoptotic cells with low mitochondrial
membrane potential, JC-1 remains a monomer, which emits green fluorescence. The isolate
is then exposed to a peptide comprised of the BH3 domain of phorbol-12-myristate-13-acetate-induced
protein 1 (PMAIP1; NOXA), which specifically binds to and inhibits the antiapoptotic
activity of MCL1. Then the mitochondrial outer membrane permeability (MOMP) is assessed
by determining the ratio of red and green JC-1 fluorescence.;
NCI Metathesaurus CUI : CL522085;
PubMed : 22230093;
Origin ID : C134502;
UMLS CUI : C4525190;
Semantic type(s)
- concept_is_in_subset
- related_to_genetic_biomarker
