NCIt related terms : CLIP-Seq; CLIP SEQUENCING; HITS-CLIP;
NCIt definition : A molecular biology method combining crosslinking-immunoprecipitation and high-throughput
sequencing techniques to detect and map RNA-protein interactions. In CLIP, whole tissues,
organisms or individual cell types are treated with UV irradiation, which introduces
covalent bonds between RNA-protein complexes. This is followed by immunoprecipitation
and removal of the protein component of the crosslinked complex. The purified RNAs
are then fragmented, reduced in size and subjected to high-throughput sequencing for
the identification of RNA binding sites.;
Alternative definition : CDISC: A molecular biology method combining crosslinking-immunoprecipitation and high-throughput
sequencing techniques to detect and map RNA-protein interactions.;
Currated CISMeF NLP mapping