Preferred Label : Bisulfite Sequencing;
NCIt synonyms : DNA Methylation Sequencing; BS-Seq; Bisulphite Conversion Followed by Sequencing; Methyl-C-Seq; MethylC-Seq; Methylation Sequencing;
NCIt related terms : Bisulfite-Seq (sequencing following treatment of DNA with bisulfite to convert cytosine
residues to uracil); Bisulfite-Seq;
NCIt definition : A DNA sequencing technique that can differentiate cytosine from 5-methylcytosine in
a DNA sample. First, a denatured DNA sample is treated with bisulfite which converts
non-methylated cytosine to uracil. Next, the sample is amplified using a PCR method
that does not discriminate between non-methylated and methylated sequences. The amplified
DNA is subjected to nucleotide sequencing. The resulting sequence is compared to an
identical control sample of DNA that was not treated with bisulfite. Unmethylated
cytosines will be displayed as cytosines in the control sample and as thymines in
the bisulfite-treated sample.;
Origin ID : C106054;
UMLS CUI : C3831347;
Automatic exact mappings (from CISMeF team)
- Semantic type(s)
- concept_is_in_subset
