ChIP-On-Chip - CISMeF

Preferred Label : ChIP-On-Chip;

NCIt synonyms : ChIP-Chip; Chromatin Immunoprecipitation-On-Chip;

NCIt definition : A molecular genetic technique that combines chromatin immunoprecipitation (ChIP) with DNA microarray technology to find candidate binding sites of DNA-associated proteins in a biological specimen. First, crosslinked protein-DNA complexes are isolated using ChIP. Next, the crosslinks are broken, the proteins are removed and the purified DNA is amplified, denatured and labeled with a fluorescent tag. The labeled, denatured DNA fragments are applied to a DNA microarray where they can hybridize to their complementary DNA sequence. After hybridization occurs, the microarray chip is imaged and the captured fluorescence signals from the array are normalized and subjected to statistical analysis. Finally, validated positive spots on the microarray can predict which DNA sequences interact with the protein of interest.;

Details

Origin ID

C106050;

UMLS CUI

C3831112;

Automatic exact mappings (from CISMeF team)
- ChIP-on-chip [EDAM topic]
Currated CISMeF NLP mapping
- ChIP-Chip [MeSH concept]
Semantic type(s)
- Molecular Biology Research Technique [UMLS semantic type]

Keyword's position in hierarchy(ies) :

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