Preferred Label : mice, knockout;
MeSH definition : Strains of mice in which certain GENES of their GENOMES have been disrupted, or knocked-out.
To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of
the gene being studied is altered to prevent synthesis of a normal gene product. Cloned
cells in which this DNA alteration is successful are then injected into mouse EMBRYOS
to produce chimeric mice. The chimeric mice are then bred to yield a strain in which
all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL
ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of
the genes.;
MeSH synonym : mice, knock-out; knock-out mice; mice, knock out; mouse, knockout; knockout mouse; knockout mice;
MeSH annotation : NIM no qualif when exper animal; do not forget also to check MICE;
Origin ID : D018345;
UMLS CUI : C0206745;
Allowable qualifiers
- Currated CISMeF NLP mapping
- Metaterm(s)
- Record concept(s)
- See also
- Semantic type(s)
- UMLS correspondences (same concept)
Strains of mice in which certain GENES of their GENOMES have been disrupted, or knocked-out.
To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of
the gene being studied is altered to prevent synthesis of a normal gene product. Cloned
cells in which this DNA alteration is successful are then injected into mouse EMBRYOS
to produce chimeric mice. The chimeric mice are then bred to yield a strain in which
all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL
ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of
the genes.
