Preferred Label : negative staining;
MeSH definition : The technique of washing tissue specimens with a concentrated solution of a heavy
metal salt and letting it dry. The specimen will be covered with a very thin layer
of the metal salt, being excluded in areas where an adsorbed macromolecule is present.
The macromolecules allow electrons from the beam of an electron microscope to pass
much more readily than the heavy metal; thus, a reversed or negative image of the
molecule is created.;
MeSH synonym : staining, negative;
Wikipedia automatic translation : Negative stain;
Wikipedia link : https://en.wikipedia.org/wiki/Negative stain;
Origin ID : D016624;
UMLS CUI : C0085194;
Allowable qualifiers
- Record concept(s)
- Semantic type(s)
The technique of washing tissue specimens with a concentrated solution of a heavy
metal salt and letting it dry. The specimen will be covered with a very thin layer
of the metal salt, being excluded in areas where an adsorbed macromolecule is present.
The macromolecules allow electrons from the beam of an electron microscope to pass
much more readily than the heavy metal; thus, a reversed or negative image of the
molecule is created.
