Preferred Label : CRISPR-Associated Protein 9;
MeSH definition : An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS
PYOGENES and other bacteria where it participates in an adaptive immune function to
cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists
of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease
module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand,
and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA
target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6
nucleotide DNA sequence immediately following the sequence targeted by Cas9.;
MeSH synonym : CRISPR Associated Protein 9; Cas9 Enzyme; Enzyme, Cas9; Cas9 Protein; Cas9 Endonuclease; Endonuclease, Cas9;
Wikipedia automatic translation : Cas9;
Registry Number MeSH : EC 3.1.-;
Wikipedia link : https://en.wikipedia.org/wiki/Cas9;
Origin ID : D000076987;
UMLS CUI : C4704710;
Allowable qualifiers
- Automatic exact mappings (from CISMeF team)
- Record concept(s)
- Related MeSH Supplementary Concept(s)
- See also
- Semantic type(s)
- UMLS correspondences (same concept)
An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS
PYOGENES and other bacteria where it participates in an adaptive immune function to
cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists
of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease
module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand,
and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA
target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6
nucleotide DNA sequence immediately following the sequence targeted by Cas9.
