LOINC display name : Asialoganglioside GM1 IgG IgM IA Qn (S);
LOINC long common name : Asialoganglioside GM1 IgG IgM Ab [Units/volume] in Serum by Immunoassay;
LOINC short name : GM1 Asialo IgG IgM Ser IA-aCnc;
LOINC description : After washing off unbound substances, a horseradish-peroxidase (HRP) labeled antibody
mix against human IgG and IgM is added to the wells and incubated for another two
hours. Following a second washing step in which unbound antibody-enzyme reagent is
removed, a substrate solution containing tetramethylbenzidine (TMB) is added to the
wells. A blue color develops in proportion to the amount of antiganglioside autoantibodies
bound to the microplate in the initial step. Color development is stopped by adding
an acidic stop solution (diluted sulfuric acid) which turns the blue solution into
yellow. The intensity of the color is measured at 450 nm. The measured absorbance
is proportional to the titer of anti-ganglioside antibodies present in a given sample.
The titers of anti-ganglioside autoantibodies are expressed as ratios of the calibrator
and can be assigned to titer categories. Sample reports are quantitative.;
Has component