Preferred Label : Carbapenemase:Type:Pt:Isolate:Nom:Carbapenemase Nordmann-Poirel;
LOINC status : ACTIVE;
LOINC display name : Carbapenemase Carba NP Nom (Isol);
LOINC long common name : Carbapenemase [Type] in Isolate by Carba NP;
LOINC short name : Carbapenemase Islt;
LOINC description : The Carba NP (Carbapenemase Nordmann-Poirel) test rapidly ( 2 hrs) detects carbapenemase
production in isolates of Enterobacteriaceae and Pseudomonas aeruginosa. Carbapenemases
are detected and can be differentiated by the CarbaNP test when the test isolate produces
the enzyme (in the absence of a chemical inhibitor) that hydrolyzes imipenem (a carbapenem
antibiotic) into the carboxylic form of the antibiotic, thereby lowering the pH of
the reaction solution which contains phenol red indicator. The acidification changes
the red color of the reaction solution to orange-yellow color (positive result) that
can be visualized easily with the naked eye. Carba NP testing can detect the following
carbapenemase types: Klebsiella pneumoniae carbapenemase (KPC; Ambler class A); Verona
integron-encoded metallo-β-lactamase (VIM), imipenemase (IMP), New Delhi metallo-β-lactamase
(NDM) (all Ambler class B); and oxacillinase-48 (OXA-48; Ambler class D). For this
test, each test isolate is inoculated into 4 reaction wells or tubes: one without
any antibiotic or chemical inhibitor against carbapenemases, one containing imipenem
without any chemical inhibitor, one containing impenem and tazobactam (which inhibits
KPC carbapenemases), one containing imipenem and EDTA (which inhibits VIM, IMP, and
NDM carbapenemases). Based on the presence and absence of color change among the 4
reaction wells or tubes, one can differentiate among the 3 classes of carbapenemases.
However, differentiation is not possible with this test in isolates that produce more
than one class of carbapenemases simultaneously.;
Origin ID : 74676-8;
UMLS CUI : C3847247;
Has component
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- Has property
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- Has time aspect
- Semantic type(s)
