" /> Mycobacterium tuberculosis stimulated gamma interferon & spot count panel:-:Pt:Bld:-: - CISMeF





Preferred Label : Mycobacterium tuberculosis stimulated gamma interferon & spot count panel:-:Pt:Bld:-:;

LOINC status : ACTIVE;

LOINC display name : M. tuberculosis stim IFN-g and spot count panel (Bld);

LOINC long common name : Mycobacterium tuberculosis stimulated gamma interferon and spot count panel - Blood;

LOINC short name : M TB IFN-g spot Pnl Bld;

LOINC description : Interferon gamma release assays (IGRAs) are used for the detection of M. tuberculosis. This panel was created for, but not limited in use to, Oxford Immunotec's T-SPOT.TB assay, which uses enzyme-linked immunospot (ELISPOT) methodology to enumerate M. tuberculosis-sensitized T cells by capturing interferon-gamma (IFN-y) in the vicinity of T cells in the patient's whole blood. Cells are spun and, using the ficoll centrifugation procedure, the polys and RBCs layer together in the bottom fraction with peripheral blood mononuclear cells (PBMCs) in the middle. The PBMCs are pipetted out to yield about 250,000 PBMCs per aliquot. They are incubated with M. tuberculosis specific antigens (ESAT-6, CFP10) in two separate wells, a phytohemagglutinin (PHA) control (a mitogenic stimulator) in another well, and a negative control in a fourth well. Incubation with the antigens allows for stimulation of any sensitized T cells present. Evaluating the number of antigen incubated spots compared to the positive and negative control spots provides a measurement of the abundance of M. tuberculosis sensitive T cells in the patient's blood.;

Details


Keyword's position in hierarchy(ies) : arborescence

You can consult :


Nous contacter.
28/04/2025


[Home] [Top]

© Rouen University Hospital. Any partial or total use of this material must mention the source.