Preferred Label : radiative energy transfer;
IUPAC definition : Transfer of excitation energy by radiative deactivation of a donor molecular entity
and reabsorption of the emitted radiation by an acceptor molecular entity.;
Scope note : the fraction aof photons emitted by d and absorbed by a is given by a
1 fd0 ¿ ¿ i¿d ¿ 1 10
¿a ¿ ca l wherecais the molar concentration of
acceptor, fd0is the fluorescence quantum yield in the absence of acceptor, l is the
thickness of the sample, i¿d ¿and ¿a ¿ are the spectral distribution of the
spectral radiant intensity of the donor fluorescence and the molar decadic absorption
coefficient of the acceptor, respectively, with the normalization condition fd0
¿ ¿ i¿d ¿ .for relatively low absorbance, a can be approximated
by a 2.3 fd0 ca l ¿ ¿
i¿d ¿ ¿a ¿ where the integral represents the overlap between the
donor fluorescence spectrum and the acceptor absorption spectrum.; radiative energy transfer depends on the shape and size of the vessel utilized and
on the configuration of the latter with respect to excitation and observation.; radiative transfer results in a decrease of the donor fluorescence intensity in the
region of spectral overlap. such a distortion of the fluorescence spectrum is called
inner-filter effect.;
Origin ID : R05063;
See also
Transfer of excitation energy by radiative deactivation of a donor molecular entity
and reabsorption of the emitted radiation by an acceptor molecular entity.
