Preferred Label : hold-up volume;
Detailed label : hold-up volume (time) in column chromatography, VM, tM;
IUPAC definition : The volume of the mobile phase (or the corresponding time) required to elute a component
the concentration of which in the stationary phase is negligible compared to that
in the mobile phase. In other words, this component is not retained at all by the
stationary phase. Thus, the hold-up volume (time) is equal to the retention volume
(time) of an unretained compound. The hold-up volume (time) includes any volumes contributed
by the sample injector, the detector, and connectors. \[t_{{M}} \frac{V_{{M}}}{F_{{c}}}\]
In gas chromatography this term is also called the gas hold-up volume (time). The
corrected gas hold-up volume (VMo) is the gas hold-up volume multiplied by the compression
(compressibility) correction factor (j): \[V_{{M}} {{o}} V_{{M}}\ j\] Assuming that
the influence of extra column volume on VM is negligible, \[V_{{M}} {{o}} V_{{G}}\];
Origin ID : H02833;
See also
The volume of the mobile phase (or the corresponding time) required to elute a component
the concentration of which in the stationary phase is negligible compared to that
in the mobile phase. In other words, this component is not retained at all by the
stationary phase. Thus, the hold-up volume (time) is equal to the retention volume
(time) of an unretained compound. The hold-up volume (time) includes any volumes contributed
by the sample injector, the detector, and connectors. \[t_{{M}} \frac{V_{{M}}}{F_{{c}}}\]
In gas chromatography this term is also called the gas hold-up volume (time). The
corrected gas hold-up volume (VMo) is the gas hold-up volume multiplied by the compression
(compressibility) correction factor (j): \[V_{{M}} {{o}} V_{{M}}\ j\] Assuming that
the influence of extra column volume on VM is negligible, \[V_{{M}} {{o}} V_{{G}}\]
